Journal: Nature Communications

Article Title: FGFR2 is essential for salivary gland duct homeostasis and MAPK-dependent seromucous acinar cell differentiation

doi: 10.1038/s41467-023-42243-0

Figure Lengend Snippet: a UMAP showing E12 SMG epithelium grouped into endbud (clusters 0, 3 and 4) and duct (clusters 1 and 2) based on Sox10 and Krt5 expression, respectively. b Violin plots showing enrichment of Fgfr1 , Fgfr2 , Tfap2a (Crect), and Krt14 in Sox10+ endbud and Krt5+ duct. c) Strategy for Fgfr1 and Fgfr2 deletion in SG epithelium. Created using Biorender.com. d) Gross images of Crect; Fgfr1 fl ;Fgfr2 fl embryos at E16. Crect + ;Fgfr1 fl + ;Fgfr2 fl/fl embryos failed to develop limbs (arrows) and had cleft palate and maxillar and mandibular hypoplasia. This was exacerbated upon the loss of an additional Fgfr1 allele. Embryos that are heterozygous for Fgfr2 were grossly normal and indistinguishable from Crect- littermate controls. e Deletion of Fgfr1 and one copy of Fgfr2 was comparable to WT glands, while Fgfr2 deletion in Crect+ epithelial cells impede SMG and SLG development. Representative images show Cre + : green, Cre-: blue, SOX10: magenta. Scale bars: 50 µm. f Deletion of Fgfr1 in Krt14+ cells did not affect endbud formation, while Fgfr2 deletion led to loss of Sox10+ endbud cells. Representative images show Cre + : green, Cre-: blue, SOX10: magenta. Scale bars: 50 µm.

Article Snippet: After blocking with 10% Normal Donkey Serum for 1 hr at room temperature (RT), sections were incubated with primary antibody ON at 4 °C (SOX10 1:100, Santa Cruz Biotechnology, sc-17342) followed by secondary antibody incubation for 1 hr at RT (Alexa Fluor® 647 AffiniPure F(ab’) 2 Fragment Donkey Anti-Goat IgG (H + L), Jackson Immunoresearch Laboratories, #705-606-147).

Techniques: Expressing

Journal: Cell

Article Title: Cell-Type-Specific Intralocus Interactions Reveal Oligodendrocyte Mechanisms in MS

doi: 10.1016/j.cell.2020.03.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary antibodies used were as follows: Sox10 (R&D Systems; 1:100), OLIG2 (MilliporeSigma; 1:1000), NF (Covance; 1:1000).

Techniques: Recombinant, Knock-Out, Electron Microscopy, cDNA Synthesis, Microarray, Sequencing, Isolation, Plasmid Preparation, Software, Real-time Polymerase Chain Reaction, Transfection, Imaging, Variant Assay

Journal: Acta neuropathologica communications

Article Title: Characterising cellular and molecular features of human peripheral nerve degeneration.

doi: 10.1186/s40478-020-00921-w

Figure Lengend Snippet: Fig. 3 Immunohistochemical and RT-qPCR analysis of Schwann cells in healthy and denervated human nerves. a-c represent nerve cross sections immunostained for SOX10 (brown) and P75 p75NTR (red) along with haematoxylin and eosin stain. The black arrow in the micrographs indicates a SOX10/p75NTR positive Schwann cell. d–i the x-axis represents Log (denervation time in days). In d-g, the horizontal black dotted line represents the mean value obtained for the healthy nerve group. a Healthy sural nerve b Biceps branch of the musculocutaneous nerve denervated for 30 days. The brown staining represents a SOX10 positive nucleus whilst the red cytoplasmic staining represents p75NTR positive staining. c Axillary nerve denervated for 294 days with deteriorated morphology. d Scatter plot to represent the total number of haematoxylin positive cells/mm2 in denervated samples. e Nonparametric smoothing linear regression of the total number of haematoxylin positive cells/mm2

Article Snippet: For the SOX10/p75NTR co-stain, the SOX10 primary antibody was added first (rabbit monoclonal EP268, CellMarque 383R-15, 1:200 for 15 min) followed by p75NTR (rabbit polyclonal, Novus Biologicals NBP1–85769, 1:400 for 30 min).

Techniques: Immunohistochemical staining, Quantitative RT-PCR, H&E Stain, Staining

Journal: Acta neuropathologica communications

Article Title: Characterising cellular and molecular features of human peripheral nerve degeneration.

doi: 10.1186/s40478-020-00921-w

Figure Lengend Snippet: Fig. 5 Immunohistochemistry and RT-qPCR analysis of p75NTR in healthy and denervated human nerves. a-c represent nerve cross sections immunostained for SOX10 (brown) and P75 p75NTR (red) along with haematoxylin and eosin stain. The black arrow in the micrographs indicates a SOX10/p75NTR positive Schwann cell. The x-axis in d–g represents Log (denervation time in days). In d and e the horizontal black dotted line represents the mean value obtained for the healthy nerve group. a Healthy sural nerve b Biceps branch of the musculocutaneous nerve denervated for 30 days. The brown staining represents a SOX10 positive nucleus whilst the red cytoplasmic staining represents p75NTR positive staining. c Axillary nerve denervated for 294 days with deteriorated morphology. d A scatter plot to represent the total number of p75NTR positive Schwann cells/mm2 in denervated samples. e Nonparametric smoothing linear regression of the total number of p75NTR positive Schwann cells/mm2. f RT-qPCR analysis of p75NTR mRNA expression across denervated samples. g Nonparametric smoothing linear regression of the p75NTR RT-qPCR data. Case numbers are attached to each data point for reference to Table 1 with descriptors of whether the samples were collected proximally or distally: m1 - Proximal part of the denervated stump of the biceps branch of musculocutaneous nerve. m2 - Distal part of the denervated stump of the biceps branch of musculocutaneous nerve. b1 - Proximal section of the denervated stump of the brachialis branch of musculocutaneous nerve. s1 - Denervated stump of suprascapular nerve. sa1 - Proximal section of the denervated stump of the spinal accessory nerve. sa2 - Distal section of the denervated stump of the spinal accessory nerve

Article Snippet: For the SOX10/p75NTR co-stain, the SOX10 primary antibody was added first (rabbit monoclonal EP268, CellMarque 383R-15, 1:200 for 15 min) followed by p75NTR (rabbit polyclonal, Novus Biologicals NBP1–85769, 1:400 for 30 min).

Techniques: Immunohistochemistry, Quantitative RT-PCR, H&E Stain, Staining, Expressing

Journal: Scientific Reports

Article Title: Single cell RNA seq reveals the pro-regenerative phenotype of thrombospondin-2 deficient dermal fibroblasts

doi: 10.1038/s41598-025-15839-3

Figure Lengend Snippet: Sox10 + population resembles Schwann Precursors with increased Pdgfb expression in TSP2 KO group. (A) Heatmap of highly differential genes (score ≥ 6, |power| > 1, gene symbols labeled with * are associated with pro-angiogenesis), (B) Violin plots, and (C) GO pathway analysis of highly regulated genes between WT and KO Schwann Precursors.

Article Snippet: The following primary antibodies were used: Sox10 Antibody [Alexa Fluor 700] (1:200, Novus Biologics, NBP2-59621AF700), anti-Neurofilament H (NFH) (1:200, EMD Millipore, AB1989), anti-Vimentin (1:500, EMD Millipore, AB5733), Sox10 Rabbit pAb (1:100, ABclonal, A15100).

Techniques: Expressing, Labeling

Journal: Scientific Reports

Article Title: Single cell RNA seq reveals the pro-regenerative phenotype of thrombospondin-2 deficient dermal fibroblasts

doi: 10.1038/s41598-025-15839-3

Figure Lengend Snippet: TSP2 KO back skin wounds demonstrate increased neurogenesis with increased Sox10 + population and Pdgfb expression in TSP2 KO DFs. (A) Flow cytometry of Sox10 in isolated primary DFs and (B) quantification of Sox10+ (%) cells. (C) qPCR of Pdgfb in WT and TSP2 KO DFs. (D) Immunofluorescence staining of NFH and Sox10 with Vimentin in D7 WT and TSP2 KO mouse back skin wounds and quantification of (E) number of nerve bundles (NBs) in the center, (F) diameter of NBs (µm), and (G) Sox10 + area/HPF (%) across the whole wounds (dashed circles indicate nerve bundles, n = 3–4, Unpaired T-test, two-tailed, *, p < 0.05, **, p < 0.01).

Article Snippet: The following primary antibodies were used: Sox10 Antibody [Alexa Fluor 700] (1:200, Novus Biologics, NBP2-59621AF700), anti-Neurofilament H (NFH) (1:200, EMD Millipore, AB1989), anti-Vimentin (1:500, EMD Millipore, AB5733), Sox10 Rabbit pAb (1:100, ABclonal, A15100).

Techniques: Expressing, Flow Cytometry, Isolation, Immunofluorescence, Staining, Two Tailed Test